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Organoleptické defekty vína zpôsobené plesňami
Peško, Jakub
This thesis dealt with the issue of an impact of mildew on wine quality. It clarifies the negative effects of mold which already occur in vineyard on grape bunches and the rest of the vine bush. It also explains that the mold is not getting into the wine only from mold infected grapes but also during the wine making and wine improving in cellar management. The thesis analyzes the characteristics and impacts of mold secondary metabolites and their wine quality affecting changes. It further describes a method of determination of certain substances that affect organoleptic wine characteristics and even threaten a human health.
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Porovnání metod stanovení obsahu mykotoxinů v obilninách
Boving, Veronique
In the Bachelor thesis I have dealt with a comparison of methods for determination of mycotoxins in cereals. The first part is a recent survey, in which I described the distribution of mycotoxins, I described the toxigenic fungi and produced mycotoxins. I noted the characteristics of the best-known mycotoxin and described some of the analytical method for the determination of mycotoxins. In the second part I focused on analysis of cereal samples. A total of 12 samples were analyzed by parallel analysis, that should determine the accuracy of the measurement. Analysis was performed using three methods of analysis, and ROSA for ZEA and DON, Reveal Q + for DON DON DON RAPID, Veratox for ZEA and the reference method was HPLC method. Difference was observed in the parallel measurements, the results of mycotoxins analyzed by various methods differed. We can not say which method is more accurate.
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Studium vlivu mykotoxinů ve výživě zvířat
SUKOVÁ, Denisa
This bachelor thesis deals with mycotoxins, their impact on health and productivity of farm animals. Mycotoxins are toxic secondary metabolites of fungi. The introductory part of the thesis focuses on the basic fungi and mycotoxin characteristics. Further, the classification of mycotoxins is described. A significant part of this thesis is made of the characteristics of individual mycotoxins and their impact on health and productivity of farm animals. Great emphasis is put on the prevention of formation and decontamination of mycotoxins in animal food. The conclusion mentions legislation as well as limits
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Effects of alpha-zearalenol on hyperactivation and acrosomal reaction of the boar sperms
Doleželová, Kateřina ; Rajmon, Radko (advisor) ; Marie, Marie (referee)
Alpha zearalenol is a derivative of the mycotoxin zearalenone, which is commonly found in agricultural crops and commercial feed. It is a secondary metabolite of filamentous fungi of the genus Fusarium. The alpha zearalenol is the result of a biotransformation of zearalenone in the liver and intestinal tissue. When the zearalenone is metobolised this way, it is able to compete for the binding sites of estrogen receptors because the structure is very similar to 17 beta estradiol.
Toxicity of alpha zearalenol therefore lies in an estrogenic stimulation, which induces morphological and functional changes in the reproductive organs and gametes. Toxicity is manifested on the male gamete by reducing viability, motility and the portion of sperms capable of undergoing acrosome reaction.
The aim of this study was to evaluate the effect of alpha zearalenol on the hyperactivation and acrosome reaction of boar sperm depending on the concentration and duration of exposure to mycotoxins.
For the purposes of this study we used two methodologies and the short term chilled commercial insemination doses which were kept in a box with the temperature of 17 degrees of Celsius. The two methodologies had a different count of used concentrations of alpha zearalenol and exposure time. Acrosome reaction of sperms was assessed in both methodologies, while the share of hyperactive sperm was assessed only in the second methodology.
The first methodology did not prove any effect of alpha zearalenol on acrosome reaction, but the second methodology has proven a positive effect of concentrations 5, 10 and 20 uM alpha zearalenol on acrosome reaction of boar sperm. Individual values of parameters from CASA analysis (VCL, ALH, LIN and WOB), which were used for evaluation of hyperactivation of boar sperm, were not affected by any used concentrations of alpha zearalenol.
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Silážování vojtěšky seté
Zitta, Jan
ABSTRACT The goal of this bachelor thesis is to determine the proper procedure for silage of lucerne sown. The first part of the bachelor thesis deals with the description of lucerne sown, her morphological structure and claims in nutrients. Lucerne sown is undemanding to add nutrients to the soil, but before sowing it can be fertilized for stock. Bachelor thesis deals also with soil and climatic conditions on the quality of the fodder and her feed value. To ensure optimal production with high forage quality it is important to have an optimum ratio of air and water in the soil. The second part of bachelor work explores the meaning of silage, technological methods for the production of silages and of silage influence on the quality and health safety forage of lucerne sown. During silage it is important to have quick acidification of the mass, thereby suppressing undesirable influence of microflora, which causes depreciation of the silage. For silage of Lucerne sown are suitable especially chemical silage additives, which are very important for quick acidification of the silage mass.
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Porovnání metod stanovení obsahu mykotoxinů v obilninách
Boving, Veronique
In the Bachelor thesis I have dealt with a comparison of methods for determination of mycotoxins in cereals. The first part is a recent survey, in which I described the distribution of mycotoxins, I described the toxigenic fungi and produced mycotoxins. I noted the characteristics of the best-known mycotoxin and described some of the analytical method for the determination of mycotoxins. In the second part I focused on analysis of cereal samples. A total of 12 samples were analyzed by parallel analysis, that should determine the accuracy of the measurement. Analysis was performed using three methods of analysis, and ROSA for ZEA and DON, Reveal Q + for DON DON DON RAPID, Veratox for ZEA and the reference method was HPLC method. Difference was observed in the parallel measurements, the results of mycotoxins analyzed by various methods differed. We can not say which method is more accurate.
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Organoleptické defekty vína zpôsobené plesňami
Peško, Jakub
This thesis dealt with the issue of an impact of mildew on wine quality. It clarifies the negative effects of mold which already occur in vineyard on grape bunches and the rest of the vine bush. It also explains that the mold is not getting into the wine only from mold infected grapes but also during the wine making and wine improving in cellar management. The thesis analyzes the characteristics and impacts of mold secondary metabolites and their wine quality affecting changes. It further describes a method of determination of certain substances that affect organoleptic wine characteristics and even threaten a human health.
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Mykotoxiny v potravinách a jejich vliv na zdraví člověka
Jiráková, Hana
This bachelor thesis is a literature review dealing with the occurrence of mycotoxins in foods and their effect on human health. The first part is devoted to the basic characteristics of selected filamentous fungi and their metabolic products adversely affecting human health. Mycotoxins are toxic and carcinogenic substances produced mainly by fungi of the genera Aspergillus, Penicillium and Fusarium. The second part describes the impact of mycotoxins on human health and identifies appropriate measures to fight against mycotoxins. The last part is devoted to describing the actual situation in the Czech Republic.
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